Facility
Proteomass Facility- Laboratory for Biological Mass Spectrometry “Isabel Moura”
Laboratory Director
Dr. Hugo Miguel Baptista Carreira dos Santos Ph.D. | MRSC
https://linktr.ee/HugoM.Santos
- Assistant Researcher – FCT Investigator Program at LAQV-REQUIMTE-NOVA-FCT-Portugal
- Adjunct Assistant Professor of Pathology-School of Medicine | University of Pittsburgh-USA
- Editorial Advisory Board of Talanta – Elsevier
Hugo Miguel Baptista Carreira dos Santos
Contact email: hmsantos@fct.unl.pt | hmsantos@me.com
http://www.researcherid.com/rid/D-1036-2011
http://orcid.org/0000-0002-6032-8679
https://www.researchgate.net/profile/Hugo_Santos/
His research is mainly focused in the development of new sample treatments for proteomics, analytical chemistry and bio-analytical chemistry. Briefly, he is involved in the development of new approaches for in-gel and in-solution protein identification throughout ultra-fast protein digestion and MALDI Imaging.
Recently he was involved in a collaboration for the development of new strategies for tissue sample preparation prior Mass Spectrometry Imaging of endometriosis tissues, prostate cancer and tissues. His PhD was supervised by Dr. José Luis Capelo (University of Vigo), Prof. José Moura (Universidade NOVA de Lisboa, Portugal), Dr. Garry Corthals (Turky Centre for Biotecnology, University of Turku, Finland). Dr. Santos was a postdoctoral.
Dr. Santos was a postdoctoral fellow at Universidade de Tras-os-Montes e Alto Douro (Portugal) and in the Universidade NOVA de Lisboa, FCT-REQUIMTE (Portugal).
Actually Dr. Santos is an IF Researcher working in Nano-PhosphoProteomics at the BIOSCOPE group LAQV-REQUIMTE, being responsible for the Laboratory for Biological Mass Spectrometry & Functional Associations of Post-Translational Modifications at the Labs 011- 013.
Services
Proteomics & Bio–analytical Services
- We deliver scientific counselling in clinical and analytical proteomics.
- We deliver scientific counselling in clinical biochemistry.
- Two Ultra-high resolution mass spectrometers LC-ESI-qQ-TOF MS/MS Impact HD (Bruker)
- MALDI-TOF MS Ultraflex II (Bruker)
- Label-free protein quantification by LC-ESI-qQ-TOF MS/MS (Impact HD, Bruker)
- 1D-GE SDS-PAGE
- Off-gel IEF (Micro-rotofor Cell, BioRad)
- Two-dimensional gel electrophoresis (2D-GE)
- Gel imaging and spot picking (ProPic II)
- In-gel digestion + MS and MS/MS (Bruker Ultraflex)
- In-solution digestion + MS and MS/MS (Bruker Ultraflex)
- MS and MS/MS (Bruker Ultraflex)
- Purification and concentration of peptides (Millipore® ZipTips)
- Reverse-phase Liquid Chromatography-MALDI-TOF/TOF (Accela 600, Thermo + SunChrom MALDI Spotter + Bruker Ultraflex)
- Liquid Chromatography DAD detection (Accela 600,Thermo)
- Speed vacuum for sample concentration/ evaporation
- 96 well plate assays / ELISA / protein quantification (Labtech LT – 4000 MS and CLARIOstar BMG Labtech)
Bio–Molecular, and NANO–Material Spectroscopy
- NanoDrop 1000 spectrophotometer
- NanoDrop 3300 spectrofluorimeter
- UV-vis studies
- In-solution and solid-sate emission studies. Steady and Excited states
- Nanoparticle synthesis and preparation
- 96 well plate assays / cations / anions / aminoacid quantification ( CLARIOstar BMG Labtech)
- DLS characterization (charge and size)
Prices
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